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Antibodies bound to the blocking peptide no more bind to the epitope on the focus on protein. By comparing the staining within the blocked antibody versus the antibody on your own, you can see which staining is certain.

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This tutorial outlines additional doable optimization of mobile and immunoassay parameters to be sure the very best outcomes are...

This tutorial outlines additional attainable optimization of mobile and immunoassay parameters to ensure the absolute best effects are...

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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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The measurement of protein phosphorylation is a get more info useful tool for measuring the modulation of receptor activation by equally antibodies...

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Blocking peptides are peptides that bind precisely on the focus on antibody and block antibody binding. These peptide generally incorporates the epitope recognized from the antibody.

Concentrate on Information and facts Cyclin E belongs into the really conserved cyclin family members, whose associates are characterised by a dramatic periodicity in protein abundance through the cell cycle. Cyclins perform as regulators of CDK kinases. Distinctive cyclins show distinctive expression and degradation patterns which lead for the temporal coordination of every mitotic party. Cyclin E sorts a posh with and features as being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S period boundary and it is degraded as cells development through S period.

Overexpression of the gene is noticed in lots of tumors, which results in chromosome instability, and so may well contribute to tumorigenesis. This protein was observed to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a critical purpose in marketing cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already explained. Two further splice variants were claimed but thorough nucleotide sequence information is just not however accessible.

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